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The effect of PKCδ on Pyk2 and Akt phosphorylation by αIIbβ3-mediated outside-in signaling. Washed platelets from PKCδ -/- mice and PKCδ +/+ littermates were plated on either BSA (5 mg/mL) or fibrinogen (100 μg/mL) for 45 min and probed with anti-phospho-Pyk2 (Tyr402), anti-phospho-PKCδ (Tyr311), anti-phospho-Akt (Ser473) or anti-β-actin antibodies by western blotting. The blots are representative of three experiments and are mean ± SE. * p < 0.05.
