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. 2020 Oct 13;10:17095. doi: 10.1038/s41598-020-74105-w

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Tandem core technology and constructs used in this study: Top: Structure of tandem core protein as published by Peyret et al.²⁸. N-terminal core 1 (in dark green) is fused via a flexible linker (red) to C-terminal core 2 (yellow). MIR major immunogenic region. Bottom: The sequence of SpyTag (ST, orange) or SpyCatcher (SC, dark blue) was inserted into the MIR of the C-terminal core 2 of tHBcAg for exposed display on the spike, and the C-terminus of GFP (light green) and P24 (teal) respectively. For localization to the endoplasmic reticulum (ER) the transit peptide of Arabidopsis thaliana basic chitinase (purple) was fused to the N-terminus of GFP and tHBcAg. For ER retention a C-terminal KDEL sequence was added to GFP and tHBcAg.