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. 2020 Oct 13;11:5151. doi: 10.1038/s41467-020-18984-7

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a, b Representative 3D reconstructions of myelinated FSI from control (a) and γ2^f/f (b) mice showing biocytin (red) and MBP (green) labeling. Note that the distance from the soma to the onset of MBP signal is longer in the γ2^f/f mouse compared to the control (lines). The first branch point (open arrowhead) and an internode (solid arrowhead) are indicated. Scale bar: 40 µm. c, d Confocal images from reconstructed FSI in (a) and (b) showing internodes (solid arrowheads) and axonal branch points (open arrowheads). Note the longer MBP⁺ internode co-labeled with biocytin and the myelinated branch point in the γ2^f/f mouse. Scale bar: 10 µm. We reconstructed a total of n = 8 cells from N = 5 control mice and n = 9 cells from N = 3 γ2^f/f mice. e, f Dot plots of the distances from the soma to the first MBP signal (e) and from the soma to the first branch point (f) in control (dark red) and γ2^f/f (light red) mice (n = 8 cells from N = 5 control mice and n = 8 cells from N = 3 γ2^f/f mice; p = 0.0047 for (e) and p = 0.02 for (f), two-tailed Mann–Whitney U test). g Dot plots of the percentage of branch points with MBP per cell in control (dark red) and γ2^f/f (light red) mice for the same cells (n = 7 cells from N = 5 control mice and n = 7 cells from N = 3 γ2^f/f mice; p = 0.0095, two-tailed Mann–Whitney U test). h Dot plots of the mean internode length per cell for control (dark red) and γ2^f/f (light red) mice (n = 8 cells from N = 5 control mice and n = 9 cells from N = 3 γ2^f/f mice; p = 0.0003, two-tailed Mann–Whitney U test). Dot plots in (e–h) are presented as mean±s.e.m and dots represent data from individual FSI. i Distribution of internode lengths for the same cells in control and γ2^f/f mice. Fitted lines show Gaussian distributions in control (dark red) and γ2^f/f mice (light red) (Gaussian fit peak value: 21.68 ± 10.5 µm and 39.09 ± 13.71 µm for control and γ2^f/f mice, respectively). Note the shift towards longer values in γ2^f/f mice (D = 0.4747; p < 0.0001, two-sided Kolmogorov–Smirnov test).