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. 2020 Oct 13;10:17078. doi: 10.1038/s41598-020-74022-y

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Nitric oxide induced by BPC 157 contributed to the promoted cell migration. (a) HUVECs were stimulated with 1 μg/ml BPC 157, then the amount of nitric oxide production was assessed by the fluorescence (white spots shown in upper panel) generated by DAF-FM DA fluorescent probe. Results were calculated and shown in lower panel (*means compared with control p value < 0.05), (b) Representative photos showing the migration of HUVECs treated with vehicle, 1 μg/ml BPC 157, 50 nM hemoglobin and 1 μg/BPC 157 together with 50 nM hemoglobin. (c) Compared with vehicle, the number of cell migration in each group was determined. Data were shown as mean ± SEM of three independent experiments (*means compared in two groups, p value < 0.05).