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. 2020 Oct 13;10:17155. doi: 10.1038/s41598-020-74400-6

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Immunohistochemical analyses of FAM20C and Periostin in mouse periodontal ligament tissues. Immunolocalization for FAM20C (brown color) was present in PDL (a, b), with preferential distribution at the bone surface region (b; arrows) and bone embedded Sharpey’s fibres (b; arrow heads). Periostin was present (brown color) in PDL (c, d) with strong positive signals along the thick PDL collagen, both at the bone surface and cementum surface regions of PDL (d; arrows), and bone embedded Sharpey’s fibres (d; arrow heads). No immunoreactivities were detected when non-immune goat immunoglobulin (IgG) was used as a negative control for anti-FAM20C (e, f), and non-immune rabbit IgG for anti-Periostin (g, h). Scale bar; 200 μm (a, c, e, g), 50 μm (b, d, f, h).