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. 2020 Sep 7;21(18):6533. doi: 10.3390/ijms21186533

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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HS-5 cells confer drug resistance to OC cells. OC cisplatin sensitive cells carrying pECFP-DEVDR-Venus plasmid (A2780 Venus) cells were co-cultured with MS-5 (A,B) or HS-5 (C,D) and exposed to 5 μM RJY13 or 1.5 or 2 μM RJY13, respectively. Levels of cleaved CFP/YFP (CFP or YFP, A,C) and cleaved human PARP (cPARP, A) were determined by immunoblotting (A,C). Relative cPARP levels as well relative levels of cleaved CFP were calculated and plotted (B,D). GAPDH was used as a loading control. CC, co-culture.