Figure 3.

Differentiated 3T3-L1 cells confer drug resistance to OC cells. Murine adipocyte progenitor 3T3-L1 cells were stimulated to differentiate to white adipocytes (WAT) and the presence of fat droplets was determined using Oil red staining (A). A2780 cells exposed to platinum compounds (5 μM RJY13 or 25 μM cisplatin) were co-cultured with differentiated (WAT) and undifferentiated 3T3-L1 cells as well as MS-5 cells (B). Levels of cleaved PARP (cPARP) were monitored in the different samples by immunoblotting using human selective anti-cPARP antibody (B). α-Tubulin was used as a loading control. The experiment was repeated twice with comparable outcomes. CC, co-culture.