Figure 4.

Co-culture of MS-5 cells with OC cells blocks ERK1/2 activation. OC cells sensitive (A2780; A) and resistant (A2780CisR; A,B) to cisplatin were treated with 5 μM RJY13 (A,B), 50 nM colchicine, or 25 nM paclitaxel (B) alone or in co-culture with MS-5 cells. Levels of cleaved PARP (cPARP) and phospho-ERK1/2 (pERK1/2) were monitored. A2780 cells were treated with RJY13 (5 μM) in mono or co-culture with MS-5 cells in the presence or absence of 2 μM cobimetinib (C). α-Tubulin (A,B) and GADPH (C) were used as loading controls. CC, co-culture.