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. 2020 Sep 15;21(18):6751. doi: 10.3390/ijms21186751

Figure 4.

Figure 4

Erastin treatment differentially affects TFRC and ferritin heavy chain 1 (FTH1) protein expression in cells expressing distinct TP53 mutation types. Representative images and quantification of Western blots examining the expression of (A,B) transferrin receptor 1 (TFRC) and (C,D) ferritin heavy chain 1 (FTH1) in TP53 null H1299 cells (H1299) or H1299 cells transfected with wild-type (WT) TP53, or the indicated TP53 mutant following treatment with DMSO (control) or 10 µM erastin for 24 h (n = 3–4 per cell type and treatment). Relative expression was normalized using glyceraldehyde 3-phosphate dehydrogenase (GAPDH) as a loading control. * Denotes statistical differences from respective control, p < 0.05. Error bars indicate SEM.