Figure 5.

Protective efficacy of intranasal administration of BclA3_CTD and AZ703 against CDI in a murine model. C57BL/6 mice were nasally immunized with PBS, B. subtilis PY79 spores, purified BclA3_CTD or AZ703 spores and challenged with C. difficile R20291 spores. Mice were monitored in the following 5 days after infection for (A) Weight loss presented as the relative % of the weight to the day of infection (day 0 or D0); (B) Time of occurrence of diarrhea, presented as the relative % of diarrhea in a group to the total mice and (C) Score of diarrhea per day. Error bars are standard error of the mean. Two-way ANOVA Tukey’s multiple comparisons test (A and C); Log-rank (Mantel-Cox) test (B). Statistical significance (p < 0.05) is indicated by asterisks. * p < 0.05 and ** p < 0.01. No significance (n.s).