Skip to main content
. 2020 Sep 4;21(18):6476. doi: 10.3390/ijms21186476

Figure 5.

Figure 5

Effects of FAK inhibition on expressions and phosphorylations of AKT, GSK3β, Bif-1, LAMP1, HSP25, and p-HSP25 in KO astrocytes following KA injection. As compared to vehicle, FAK inhibitor 14 (FI14) abolishes the upregulations of p-AKT-S473, p-GSK3β-S9, Bif-1, and LAMP1, but not HSP25, levels 7 days after KA injection (red box). (A) Representative Western blot of expressions and phosphorylations of AKT, GSK3β, Bif-1, LAMP1, HSP25, and p-HSP25 in KO astrocytes. Cont, control animals; KA, KA-injected animals; Veh, vehicle; FI4, FAK inhibitor 14. (B) Quantifications of expressions and phosphorylations of AKT, GSK3β, Bif-1, LAMP1, HSP25, and p-HSP25 in KO astrocytes following KA injection (mean ± SEM; *,# p < 0.05 vs. control KO astrocytes and vehicle, respectively; n = 7, respectively). (C) Effect of P2X7R deletion on HSP25 and LAMP1 expression in the hippocampal astrocytes. As compared to vehicle, FI14 abrogates LAMP1, but not HSP25, expression in KO mice 7 days after KA injection.