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. 2020 Sep 14;9(9):1290. doi: 10.3390/foods9091290

Figure 3.

Figure 3

Evaluation of human macrophage cytotoxicity after Cfr-LEO and LEO exposure. (a) THP-1 M0 cell cytotoxicity was measured by CellTox Green Cytotoxicity Assay after 24 and 48 h of treatment with different concentrations of Cfr-LEO (0.005%, 0.01%, 0.02%, 0.05%). Values are plotted as the Relative Fluorescence Unit (RFU). Cn: untreated cells. Values are the mean ± SD of two biological replicates, each carried out in technical duplicates. (b) THP-1 M0 cell cytotoxicity was measured by CellTox Green Cytotoxicity Assay after 24 and 48 h of treatment with different concentrations of LEO (0.005%, 0.01%, 0.02%, 0.05%). Values are plotted as the Relative Fluorescence Unit (RFU). Cn: untreated cells. Values are the mean ± SD of two biological replicates, each carried out in technical triplicates.