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Thiamine (Thia) and DBT (50 µM, 25 h) do not influence the expression (A) and the activity (B) of transketolase (TKT1) in Neuro2a cells treated with PQ (0.25 mM, 24 h). The relative level of TKT1 mRNA was evaluated by real time PCR. One Unit of TKT1 activity corresponds to 1 µmol of glyceraldehyde 3-phosphate formed per minute at pH 7.5 and at 37 °C. The results are expressed as mean ± SD (n = 3). Statistical analysis was made by one-way ANOVA.
