Table 1.
Levels of 7-hydro-8-oxo-2′-deoxyguanosine (8-oxodG) and 5-hydroxycytosine (5-OHC) at the codon 176 along the exon 5 sequence of the p53 gene among hepatitis C virus (HCV)-associated hepatocellular carcinoma (HCC) patients and controls, as well as hepatocytes in vitro treated with 0.2 mM xanthine plus 5.0 mU xanthine oxidase (XO)-treated. Frequency of 3-(2-deoxy-β-d-erythro-pentafuranosyl)pyrimido[1,2-α]purin-10(3H)-one deoxyguanosine (M1dG) are reported.
| Oxidative Base Lesions at the Codon 176 of the p53 Gene and Generation of M1dG in Genomic DNA | |||||
|---|---|---|---|---|---|
| 8-oxodG (–TGC–) a | 5-OHC (–TGC–) a | M1dG | |||
| N | RI b ± SE | RI b ± SE | RAL c × 108 ± SE | ||
| Hospital Based Population | |||||
| Controls | 22 | 0.28 ± 0.06 | 0.29 ± 0.01 | 1.67 ± 0.18 | |
| HCV-HCC patients | 31 | 0.61 ± 0.40 | 0.95 ± 0.27 | 3.25 ± 0.41 | |
| Experimental Hepatocytes | |||||
| Untreated HepG2 cells | 6 | 0.20 ± 0.01 | 0.22 ± 0.02 | 0.35 ± 0.2 | |
| XO-treated HepG2 cells | 6 | 0.34 ± 0.03 | 0.44 ± 0.04 | 3.2 ± 0.3 | |
a Underlined letters indicate the residues for which base lesions are reported; b Relative Intensity (RI) ± Standard Error (SE); c Relative adduct labeling (RAL) × 108 normal nucleotides ± SE.