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. 2020 Sep 11;21(18):6656. doi: 10.3390/ijms21186656

Figure 1.

Figure 1

Effect of minor ginsenoside Rg2 and Rh1 on cell viability. (a) Cell viability of minor ginsenoside Rg2 and Rh1-treated RAW264.7 cells were measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The cells were treated with various concentration of Rg2, Rh1, and combination of Rg2 and Rh1 (0 to 50 μg/mL) for 24 h. In the combination of Rg2 and Rh1, Rg2 and Rh1 were mixed with a ratio of 1:1 (10 μg/mL of the combination contains 5 μg/mL Rg2 and 5 μg/mL Rh1, and so forth). Digitonin was used as negative control. (b,c) The production of nitric oxide was examined by Griess assay (b), and expressions of iNOS were evaluated by western blot analysis (c). (d) The ratio of phosphorylated iNOS/α-tubulin expression represents as the mean ± SEM (n = 3). * p < 0.05, *** p < 0.001 compared with control sample, # p < 0.05, ## p < 0.01, and ### p < 0.001 compared with lipopolysaccharide (LPS)-treated sample.