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. 2020 Sep 15;21(18):6764. doi: 10.3390/ijms21186764

Figure 2.

Figure 2

Protocols used for iPS cell reprogramming. Reprogramming protocols for iPS cell differentiation. Top indicates the standard protocol. This follows the protocol published by Center for iPS cell Research and application, Kyoto University. Protocol 1 (middle) and protocol 2 (bottom) are the novel protocol developed in this study. SB431542 is a selective inhibitor of the transforming growth factor beta (TGF-β) type I receptor activin receptor-like kinase ALK5. “TGF-β(+) medium” indicates that TGF-β was included in the reprogramming medium, and “TGF-β(−) medium + SB431542” indicates that the reprogramming medium contained SB431542 but did not include TGF-β. Day 1 was defined as the date of gene transfer. “20% O2 period” indicates that cells are incubated in O2 20%, and “5% O2 period” indicates that cells are incubated in O2 5%. Each “U-08” or “U-020” indicates the transfection program for the Lonza nucleofector transfection system. “Oct3/4, Sox2, Klf4, c-Myc” or “Oct3/4, p53sh, Sox2, Klf4, Lin28, L-Myc, Glis1, and TET1” are transfected genes in episomal vectors. p53sh indicates short hairpin p53.