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Cytotoxic effects of doxorubicin (Doxo) and gramicidin A (GA) on HT-29 spheroids. Spheroids were obtained after seeding 5000 cells/well and were grown for three days. On the third day of spheroid growth, different concentrations of Doxo (20, 50, 75, and 100 μg/mL) and GA (10, 20, 40, and 60 μM) were added and the effects were monitored through transmission microscopy at 24 and 48 h. The scale bar is 200 μm and is the same for all images.
