Table 1.
Experimental Agents a | ||
---|---|---|
Imetelstat | 5′-R-TAGGGTTAGACAA-NH2-3′ | |
Mismatch oligonucleotide (with 4 nucleotides mismatched to imetelstat) | 5′-R-TAGGTGTAAGCAA-NH2-3′ | |
Sense oligonucleotide (with reverse sequence of imetelstat) | 5′-AACAGATTGGGAT-R-3′ | |
Positive Control Agents | ||
Positive Control Ligand | Receptor | Ligand Concentration |
Heat-killed Listeria monocytogenes | hTLR2 | 108 cells/mL |
Poly(I:C) HMW | hTLR3 | 1 μg/mL |
Escherichia coli K12 lipopolysaccharide | hTLR4 | 100 ng/mL |
Salmonella typhimurium flagellin | hTLR5 | 100 ng/mL |
Imiquimod | hTLR7 | 1 μg/mL |
CL075 | hTLR8 | 1 μg/mL |
CpG ODN 2006 | hTLR9 | 100 ng/mL |
Abbreviations: CpG, cytosine triphosphate deoxynucleotide followed by a guanine triphosphate deoxynucleotide with a phosphodiester link between; HMW, high molecular weight; ODN, oligodeoxynucleotide; poly (I:C), polyinosinic-polycytidylic acid. a R in oligonucleotide sequences refers to the covalently bound lipophilic (palmitoyl) group.