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Cell number of osteoblasts cultured one, three, and seven days with and without electrical stimulation counted via ImageJ. Cell number is displayed relative to the control for each time point. For this experiment, 0.2V, 1.4 V, and 2.8 V groups were stimulated 3 × 45 min per day while the cont. 1.4 V group was stimulated continuously with 1.4 V. Cells grown on Ti6Al4V electrodes (a) and collagen-coated coverslips (b). n = 3.
