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. 2020 Oct 13;11(10):852. doi: 10.1038/s41419-020-03082-9

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — A Ectopically expressed RPS27L binds to endogenous FANCD2 and FANCI. H1299 cells were transfected with mock vector or FLAG-RPS27L, and subjected to immunoprecipitation (IP) with FLAG beads, followed by immunoblotting (IB) with indicated antibodies (Abs). WCE: whole-cell extract. B–D Endogenous RPS27L binds to endogenous FANCD2 and FANCI. A549 cells were harvested for IP with RPS27L (B), FANCD2 (C), or FANCI (D) Ab, and then IB with indicated Abs. E Schematic diagrams of full length and fragments of FANCD2 and FANCI constructs. The bar graphs were not drawn to scale and the red bar indicates the RPS27L-interacting region. F, G Mapping of FANCD2/FANCI binding to RPS27L. 293 cells were transfected with mock vector, indicated constructs encoding various fragments of FLAG-FANCD2 (F) or FLAG-FANCI (G) before harvesting for IP with FLAG beads, followed by IB with indicated Abs.