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. 2020 Oct 13;10:17147. doi: 10.1038/s41598-020-73100-5

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Change of the morphology of the SaOS-2 cell surface in dependence on the incubation condition within the alginate hydrogel after an incubation for 5 days. Representative images are shown; SEM. (A) In the absence of polyP in the gel the cell surface is smooth. This morphology alters after exposure of the cells to the gel containing polyP. (B) In gel containing “Ca–polyP-NP” the cell surface undulates > < ; (C) after exposure to “Ca–polyP/D-NP” the cells start to form pre-crystallites (p-crys) and (D) after incubation with “Ca–polyP/D-NP@polyP/AA-Coa” the surface is studded with crystallites (crys).