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. 2020 Oct 13;10:17091. doi: 10.1038/s41598-020-72726-9

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Representative micrographs of liver (A–C), muscle (D–F) and intestine (G–I) of juvenile barramundi after 7 weeks of experimental trials. All sections are stained with H&E with 40 magnification. Variation in villous height (J), villous width (K), microvilli height (L) and goblet cells density (M) in fish fed test diets, compared by Turkey multiple comparison test at P < 0.05. NH, normal hepatocyte; LD, lipid droplet; HM, healthy myotome; DM, myodigeneration; VH, villous height; GCs, goblet cells; VW, villous width and MVH, microvilli height.