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. 2020 Sep 6;9(9):831. doi: 10.3390/antiox9090831

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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HPLC chromatogram of Ougan PMF-rich fraction and mice intestinal content samples (λ = 280nm). (a) HPLC chromatogram of Ougan PMF-rich fraction. 1: neohesperidin; 2: poncirin; 3: isosinensetin; 4: monohydroxy-pentamethoxyflavone; 5: sinensetin; 6: tetramethyl-O-isoscutellarein; 7: nobiletin; 8: 5,4′-dihydroxyl-3,7,8,3′-tetramethoxyflavone; 9: tetramethyl-O-scutellarein; 10: tangeretin; 11: 5-demethylnobiletin. Nobiletin (7), tangeretin (10), and 5-demethylnobiletin (11) are the three major components in Ougan PMF-rich fraction. (b) HPLC chromatogram of intestinal content of the mixture of control group. (c) HPLC chromatogram of intestinal content of the mixture of all groups. 1: neohesperidin; 2: nobiletin-O-glucuronide (1); 3: tangeretin-O-glucuronide (1); 4: nobiletin-O-glucuronide (2); 5: nobiletin-O-glucuronide (3); 6: dihydroxy-tetramethoxyflavone (1); 7: dihydroxy-monomethoxyflavone; 8: dihydroxy-trimethoxyflavone; 9: dihydroxy-tetramethoxyflavone (2); 10: monohydroxy-trimethoxyflavone; 11: tangeretin-O-glucuronide (2); 12: dihydroxy-tetramethoxyflavone (3); 13: dihydroxy-tetramethoxyflavone (4); 14: nobiletin-O-glucuronide (4); 15: monohydroxy-pentamethoxyflavone (1); 16: trihydroxy-flavone; 17: monohydroxy-tetramethoxyflavone (1); 18: monohydroxy-pentamethoxyflavone (2); 19: monohydroxy-tetramethoxyflavone (2); 20: monohydroxy-pentamethoxyflavone (3); 21: dihydroxy-dimethoxyflavone; 22: monohydroxy-tetramethoxyflavone (3); 23: tetramethoxyflavone; 24: nobiletin; 25: tangeretin; 26: 5-demethylnobiletin.