Figure 2.

OE-MSC expression of different pluripotent and stem cell markers. (A) RT-PCR showing the expression of different markers for stemness and pluripotency in OE-MSCs. Expression of the housekeeping gene for β-Actin was used as a reference control. M: 1Kb ladder, C+ve: PCR reaction control using hESC H9 cell line (Nanog, Klf4, and Sox2) and neuronally differentiated SH-SY5Y cells (Sox9, Pax3, PTCH1 and TrkB), (+): cDNA used as template for PCR amplified with RT enzyme, (−): no RT enzyme. (B) Immunophenotyping of C3 and FA6 samples showed these cells are positive (red) when stained with antibodies specific for MAP1B, Tuj1, SMI31 and Nestin. DAPI staining is shown in blue. Scale bars = 50 μm.