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. 2020 Sep 8;21(18):6572. doi: 10.3390/ijms21186572

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Comparative NMR analysis of K. pneumoniae O2v2 and O2v1 O-antigens. ¹H HR-MAS NMR spectra of the lipopolysaccharides from (a) K. pneumoniae BIDMC 7B and (b) ABC152; and (c) the O-specific polysaccharides of K. pneumoniae Kp26 strain (O2v1) and (d) the PCM-27 strain (O2v2); (e) ¹H,¹³C HSQC-DEPT NMR spectrum of the BIDMC 7B strain (O2v1). The capital letters refer to protons and/or carbons of O-PS carbohydrate residues, as shown in Table 1. The symbol Nomenclature for Graphical Representation of Glycans is used for O-PS visual representation: Galactose; Galactofuranose to show O-specific polysaccharide repeating units: →3)-β-D-Galf-(1→3)-α-D-Galp-(1→ (D-galactan I, O2 variant 1) and →3)-β-D-Galf-(1→3)-[α-D-Galp-(1→4)]-α-D-Galp-(1→ (O2v2) [26].

Inline graphic — Comparative NMR analysis of K. pneumoniae O2v2 and O2v1 O-antigens. ¹H HR-MAS NMR spectra of the lipopolysaccharides from (a) K. pneumoniae BIDMC 7B and (b) ABC152; and (c) the O-specific polysaccharides of K. pneumoniae Kp26 strain (O2v1) and (d) the PCM-27 strain (O2v2); (e) ¹H,¹³C HSQC-DEPT NMR spectrum of the BIDMC 7B strain (O2v1). The capital letters refer to protons and/or carbons of O-PS carbohydrate residues, as shown in Table 1. The symbol Nomenclature for Graphical Representation of Glycans is used for O-PS visual representation: Galactose; Galactofuranose to show O-specific polysaccharide repeating units: →3)-β-D-Galf-(1→3)-α-D-Galp-(1→ (D-galactan I, O2 variant 1) and →3)-β-D-Galf-(1→3)-[α-D-Galp-(1→4)]-α-D-Galp-(1→ (O2v2) [26].