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. 2020 Sep 7;9(9):1245. doi: 10.3390/foods9091245

Figure 2.

Figure 2

Portion of the Sanger sequencing of the segment of the AHAS1C gene spanning the site of genome editing, at position 1676, highlighted in blue. This base is homozygous G (wild type) in Clearfield canola variety 5545 CL, homozygous T (mutant) in SU canola variety 40K and G/T heterozygous in SU canola varieties C5507 and C1511. The region sequenced extended from AHAS1C-specific SNVs at positions 1363 and 1381 to beyond position 1704, verifying the location of the genome edit at position 1676. The location of the primer used for sequencing is indicated in Figure 3.