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. 2020 Sep 7;9(9):1245. doi: 10.3390/foods9091245

Figure 3.

Figure 3

Sequence of part of exon 2 of the SU canola AHAS1C gene showing alignments of the primers and probe for the 334 bp amplicon. The forward primer and probe correspond to the target sequence while the reverse primer is complementary to the target sequence. The star by the forward primer indicates the G that is characteristic of the AHAS1C gene, distinguishing it from the AHAS3A gene which has a C at that site. The star on the reverse primer indicates the single SNV that confers herbicide tolerance on AHAS1C SU and AHAS3A SU and CL. The sequencing primer is the oligonucleotide use as primer for the Sanger sequencing presented in Figure 2.