Table 1.
Sequences of primers and probes used. +A denotes the locked nucleic acid (LNA) version of A; FAM, 6-carboxyfluorescein; -BHQ, Black Hole Quencher-1.
| Name | Primer Sequence (5′ to 3′) | Position | Amplicon Length | Reference |
|---|---|---|---|---|
| SU Canola-Specific Primers and Probe | ||||
| SU-Forward Primer | TCC TCG ACG AGC TAA CCG | 1364–1381 | 334 | This Study |
| SU-Reverse Primer | GCT TTG TAG AAC CGA TCT TCC +A | 1676–1697 | ||
| SU-Probe | FAM-ACA ACC AGC ATC TTG GGA TGG TCA-BHQ | 1646–1669 | ||
| Endogenous Reference Primers and Probe | ||||
| CruA-Forward Primer | GGC CAG GGT TTC CGT GAT | 1408–1425 | 101 | [39] |
| CruA-Reverse Primer | CCG TCG TTG TAG AAC CAT TGG | 1488–1508 | ||
| CruA-Probe | FAM- AGT CCT TAT GTG CTC CAC TTT CTG GTG CA-BHQ | 1427–1455 |