Table 2.
Real-time qPCR amplification of SU canola and Clearfield canola DNA with PCR systems specific for the canola endogenous reference gene CruA and for the AHAS1C-SU gene of SU canola. DNA isolated from three SU canola varieties, C1511, C5507, and 40K, and three Clearfield canola varieties, 5545 CL, CS220 CL, and 2022 CL, were tested with primers specific for the AHAS1C-SU gene or for the CruA gene, with 200 ng input DNA per reaction. PCR conditions are as described in Methods. For all conditions except water control, n = 3. ND indicates that no amplification was detected; NA indicates “Not Applicable” because no amplification was observed.
| PCR Specificity | AHAS1C-SU | CruA | ||
|---|---|---|---|---|
| Canola Variety | Mean Ct | Ct% CV | Mean Ct | Ct% CV |
| 5545 CL | ND | NA | 20.81 | 0.16% |
| CS2200 CL | ND | NA | 21.24 | 0.48% |
| 2022 CL | ND | NA | 21.02 | 0.16% |
| C5507 | 23.71 | 0.15% | 20.93 | 0.09% |
| C1511 | 23.40 | 0.08% | 20.87 | 0.10% |
| 40K | 22.37 | 0.41% | 20.55 | 0.29% |
| Water | ND | NA | ND | NA |