Table 2.

Overview of exosome isolation from different biological sample types using size exclusion chromatography (SEC). This table summarizes the recent publications that used SEC to isolate exosomes. Shown are the various biological fluids, types of SEC columns used, starting sample volume, fractions that contained exosomes, size of isolated exosomes, and type of cargo enriched in the isolated vesicles. Some of the listed studies isolated exosomes using SEC alone or in combination with other methods. All included papers performed exosome characterization in accordance with MISEV 2018 guidelines.

Sample Type	Type of Column	Sample Volume (mL)	Fractions Used	Size of Isolated Evs	Type of Cargo	References
Plasma	Sepharose CL-2B, qEV original	1–2	4–6, 8–10, 4–7, 10–12, 7–10	20–200 nm	Proteins, miRNAs	[20,21,22,23,24,25]
Serum	qEV original, Sepharose CL-2B	0.5–1	7–9, 8–10	50–200 nm	miRNAs, proteins	[26,27,28]
Milk	qEV original, Sephacryl S-500	0.5	7–10	<200 nm	RNAs	[29,30]
Urine	qEV, Sepharose CL-4B/2B	0.5–3	8–11, 9–10, 7–10, 7–19	40–200 nm	miRNAs, proteins, RNAs	[31,32,33,34]
Saliva	miniPURE-EVs, qEV	1	7–11, 8–10	50–200 nm	miRNAs, proteins	[35,36]
CSF	Exo-spin™ mini-column, qEV single	0.1–3	5–6, 3–4	30–150 nm	Protieins	[37,38]
Synovial fluid	Sephacryl S-500 HR	–	2–4	<200 nm	Proteins	[39]
Tears	qEV	1	8–10	<200 nm	Proteins	[35]
Seminal fluid	Exo-spin™ column	1	5–9	<200 nm	–	[40]
Nasal lavage	qEV original	0.5	7–9	<200 nm	miRNAs	[41]
Stromal vascular fraction; adipose tissue	qEV70s single, Illustra Sephacryl S-1000	0.15–0.7	8–11, 8–16	50–700 nm, <250 nm	miRNAs, neutral lipids	[42,43]
Conditioned media	qEV original, Sepharose CL-2B, Sepharose CL-4B	0.5–1.5	3–7, 7–9, 7–10, 6–12	30–200 nm	mRNAs, proteins, miRNAs	[41,44,45,46,47,48,49]