Figure 2.

Effect of probiotics on P. aeruginosa–activated phosphorylated Akt and NOD1 protein expression. SW480 cells were either left untreated (CON) or treated with LGG or BL, along with infection with P. aeruginosa PAO1 for 1 h. Immunoblotting was performed using whole-cell lysates using an Ab to detect phosphorylated (p) Akt and NOD1 protein expressions or GAPDH for normalisation. (a) Representative immunoblots. (b) and (c) Densitometric quantification of immunoreactive bands. Relative band densities of the phosphorylated Akt (b) and NOD1 (c) proteins in the presence of probiotics were quantified as the fold increase compared to the levels in PAO1-infected cells only. Each value represents the mean ± SEM of duplicate wells from at least three separate experiments (*P < 0.05).