Figure 3.

Involvement of Akt in the suppressive probiotic effects on the P. aeruginosa–induced IL-8 expression in SW480 cells. SW480 cells were transfected either with a control or AKT siRNA (siAKT = siRNA to AKT) for 48 h. The transfected cells were then either left non-infected or infected by P. aeruginosa PAO1 for 1 h under the treatment of LGG or BL. After the infection, total RNA samples were prepared and analysed using real-time quantitative RT-PCR to estimate the number of the IL-8 transcripts. The amount of the produced mRNA is shown as the fold increase over non-infected control cells. The corresponding amount of GAPDH is used as a normalised transcript. Results are shown as the mean ± SEM of duplicate wells from at least three separate experiments (*P < 0.05).