Figure 4.

Involvement of NOD1 in the positive probiotic effects on P. aeruginosa–induced hBD-2 in SW480 cells. SW480 cells were transfected with control or NOD1 siRNA (siNOD1 = siRNA to NOD1) for 48 h. The transfected cells were then either left non-infected or infected with P. aeruginosa PAO1 for 1 h under the treatment of LGG or BL. After the infection, total RNA samples were prepared and analysed using real-time quantitative RT-PCR to estimate the number of hBD-2 transcripts. The amount of produced mRNA is shown as the fold increase over the non-infected control cells. The corresponding amount of GAPDH is used as a normalised transcript. Results are shown as the mean ± SEM of duplicate wells from at least three separate experiments (*P < 0.05).