Figure 5.

Involvement of AKT and NOD1 in the regulated probiotic effects on IL-8 and hBD-2 protein secretion in P. aeruginosa–infected SW480 cells. SW480 cells were transfected with control siRNA and NOD2 siRNA (siRNA=non-target control siRNA; siAKT=siRNA to AKT; siNOD1=siRNA to NOD1) for 48 h. The transfected cells were left uninfected or infected with P. aeruginosa PAO1 for 1 h under the treatment of LGG or BL. Supernatant was analysed by ELISA for IL-8 and hBD-2. The amount of the produced IL-8 (a) and hBD-2 (b) is shown as the fold increase over non-infected control cells. Results are represented as the mean±SEM for at least three determinations from independent experiments (*P < 0.05).