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. 2020 Oct 14;149:104560. doi: 10.1016/j.micpath.2020.104560

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© 2020 The Authors

Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.

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Fig. 2 — Construction of IB DNA plasmids. Three DNA plasmids were constructed using a bicistronic co-expression vector pBudCE4.1. (a) The M41 S1 gene was cloned downstream of the EF1α promoter to construct the pBudM41-S1 DNA plasmid as in the top left construct (b) The CR88 S1 gene was cloned downstream of the CMV promoter to construct the pBudCR88-S1 as in the top right DNA construct (c) The co-expressing DNA plasmid pBudCR88S1/M41S1 was constructed by inserting the S1 genes of CR88 and M41 strains downstream of the EF1α and CMV promoters, respectively as depicted in the construct placed below the two individual constructs.