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COX10-deficient patient fibroblasts were transfected with AMPK-specific siRNA (siAMPK) or control siRNA (siCNT). Following transfection, cells were (A) evaluated for protein expression by western blotting, or (B) treated with PT1 or DMSO (vehicle) for an additional 6 days and then evaluated for viability by calcein-AM fluorescence assay and (C) morphology by phase contrast microscopy.
