Fig 2. Increased weight loss and decreased survival seen in VAD male mice infected with Salmonella.

Weight (g) of VAD and control male and female mice was recorded weekly (A) during growth from 4 weeks to 9 weeks (n = 26–30). Nine week-old mice were infected with S. Typhimurium D23580 via the intraperitoneal (i.p.) route and weight (g) was monitored daily (B) for up to 4 days post-infection (n = 8–9). Data are represented as mean ± SEM. Significance between weights by diet or day was determined using a Mann-Whitney test (*, p<0.05; ns, not significant). (C) Percent survival was assessed for all groups over the 4-day infection with a Kaplan-Meier survival curve (n = 8–9). Results are reported as percent survival. Pairwise comparisons between the VAD male group and each other group was determined with a survival analysis log-rank (Mantel-Cox) test (*, p<0.05). All data represent a compilation of data from three independent experiments.