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Variables and Their Levels in Box-Behnken Design
| Factors | Factor levels | ||
|---|---|---|---|
| − 1 (low) | 0 (medium) | + 1 (high) | |
| A: lecithin (mg) | 100 | 200 | 300 |
| B: chitosan (mg) | 10 | 15 | 20 |
| C: PEG 2000 (mg) | 20 | 30 | 40 |
| Dependent variables | Constraints | ||
| Y1 = entrapment efficiency (%EE) | Maximise | ||
| Y2 = particle size (nm) | Minimise | ||
| Y3 = zeta potential (mV) | Maximise | ||
