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. 2019 Oct 10;105(9):2316–2326. doi: 10.3324/haematol.2019.223024

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Copyright© 2020 Ferrata Storti Foundation

This article is distributed under the terms of the Creative Commons Attribution Noncommercial License (by-nc 4.0) which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited.

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Figure 1. — Expression of the two RAL isoforms in multiple myeloma cells. Representative Western analyses showing expression levels of RALA, RALA-GTP and RALB as well as expression and phosphorylation levels of MAPK and Akt signaling in (A) human myeloma cell line (HMCL) (n=7) and (B) primary MM samples (n=10). α- tubulin served as loading control. (C) In situ expression of RALA and RALB in bone marrow trephines of multiple myeloma (MM) patients (n=26). CD138 staining as well as RALA and RALB staining shown for 3 different patients (I, II, III) at 200x (I) and 400x (II, III) magnification. Scale bars: 50 μm. Samples I, II, and III correspond to sample numbers 22, 25, and 26 in the Online Supplementary Table S2, respectively.