Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Nov 7;105(10):2420–2431. doi: 10.3324/haematol.2019.224956

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright© 2020 Ferrata Storti Foundation

This article is distributed under the terms of the Creative Commons Attribution Noncommercial License (by-nc 4.0) which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited.

PMC Copyright notice

Figure 3. — Molecular perturbation triggered by ARQ531 in acute myeloid leukemia cells. (A) Heatmap for the highest 50 down- and up-regulated genes (P<0.0001) following ARQ531 treatment of OCI-AML3 cells. (B) Volcano plot of RNA-sequencing of drug- versus dimethylsulfoxide-treated cells in OCI-AML3 showing that 377 and 852 genes were significantly up- and down-regulated, respectively, with a fold change (FC) >2. (C) Table of the ten most significantly enriched gene sets, from the Hallmark collection, enriched with genes downregulated by ARQ531 in acute myeloid leukemia (AML) cells. Number of genes in each set (n), the normalized enrichment score (NES), and the test of statistical significance of the false discovery rate (FDR) q value are highlighted. (D) Enrichment plots of the top four most significantly enriched gene sets (MYC-related) in transcriptional profiles of AML cells treated (right) or untreated (left) with ARQ531. (E) Connectivity score generated by the LINCS L1000 Characteristic Direction Signature Search Engine tool, which compared the ARQ531-derived transcriptional profile against 10,000 “perturbagen” signatures (corresponding to shorthairpin RNA, open reading frame and compounds). Top-ranked scores of relevant results are indicated by arrows. HDACi: histone deacetylase inhibitor.