Figure 3.

Platelet accumulation in the stenosed region under shear rate gradients (SRG) is bi-phasic. Hirudinated human whole bloos was perfused through channels of microfluidic chambers coated with a fibrinogen solution (300 µg/mL). (A) Representative differential interference contrast (DIC) microscopy images of platelets accumulating on immobilized fibrinogen in the stenosed region at indicated time points. Scale bar: 10 µm. (B) Representative curves of platelet accumulation in zone 1 and zone 3 over time. (C) The bar graph represents the slope of platelet accumulation in zone 3 before and after 210 s. Values are the mean ± standard error of the mean (SEM) in eight separate experiments, *P<0.05.