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. Author manuscript; available in PMC: 2021 Aug 21.
Published in final edited form as: ACS Chem Biol. 2020 Jul 13;15(8):2079–2086. doi: 10.1021/acschembio.0c00222

Figure 2.

Figure 2.

C8 Alkyl Cysteine Causes Rapid Delocalization of NRAS from the Plasma Membrane. (a) EGFP-NRAS plasmid was stably transfected in HeLa S3 cells and treated with 20 μM C8 alkyl cysteine (1) for 30 min to visualize delocalization of NRAS from the plasma membrane. (b) Profile plots of cross sections (blue line) show EGFP-NRAS delocalization before (0 min) and after (30 min) treatment with 1. (c) EGFP-NRAS HeLa S3 cells were treated with 20 μM of the control C8 alkyl serine (2) for 30 min. (d) Profile plots of cross sections show EGFP-NRAS delocalization before (0 min) and after (30 min) treatment with 2. (e) EGFP-NRAS HeLa S3 cells were treated with 20 μM 2-bromopalmitate (2-BP) for 30 min. (f) Profile plots of cross sections show EGFP-NRAS delocalization before (0 min) and after (30 min) treatment with 2-BP. NRAS was fused to EGFP (green) and the Golgi apparatus was stained with BODIPY TR Ceramide (red). Scale bars denote 10 μm.