Figure 5.

C8 Alkyl Cysteine Reduces Proliferation and Induces Apoptosis in NRAS-Driven versus BRAF-Driven Melanoma Tumors. (a) Schematic and timeline of in vivo subcutaneous melanoma xenograft implantation and intratumoral (IT) treatments. (b) Histology was performed for BRAF-mutated melanoma tumors (Sk-Mel-28) and NRAS-mutated melanoma tumors (WM3000) for both vehicle control (123.51 mg/kg TCEP in saline) and C8 alkyl cysteine (1) treatment groups. Consecutive tissue sections were stained with hematoxylin and eosin (H&E) for tumor cell morphology, anti-Ki-67 for cell proliferation, anti-cleaved caspase-3 (CC3) for cell apoptosis, and anti-phosphorylation of ERK (p-ERK) for RAS signaling.