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. Author manuscript; available in PMC: 2021 Jan 9.
Published in final edited form as: Cell. 2019 Dec 26;180(1):188–204.e22. doi: 10.1016/j.cell.2019.11.036

Figure 1. Generation of GBOs that Retain Histologic Features of Parental Tumors.

Figure 1.

(A) A schematic of the procedure with sample bright-field images. Scale bar, 1 mm.

(B) Sample H&E staining images of parental tumors and corresponding GBOs. Age of GBOs in weeks (W) is listed. Scale bars, 20 μm.

(C) Sample confocal image of micro-vasculature retained in GBO with immunostaining for CD31. Scale bar, 100 μm.

(D) Sample confocal images showing the hypoxia gradient present in a large GBO with immunostaining for HypoxyProbe and KI67. Insets highlight KI67+ proliferating cells at the periphery (box 2), but not in the hypoxic core (box 1). Scale bars, 100 μm.

See also Figure S2 and Tables S1 and S2.