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. 2020 Oct 14;6(42):eabb5202. doi: 10.1126/sciadv.abb5202

Fig. 6. Human Lnc-UC (hLnc-UC) is a cycling and anti-inflammatory lncRNA.

Fig. 6

(A) Genomic locations of Lnc-UC and hLnc-UC. (B) CISH analysis of hLnc-UC in human colons. Scale bar, 50 μm. (C) hLnc-UC, Nlrp3, and IL-1β levels in the mucosae of patients with colitis and healthy individuals. (D) Rhythmic expressions of hLnc-UC, BMAL1, REV-ERBα, and NLRP3 in serum-shocked THP-1 cells. (E) hLnc-UC overexpression induces mRNA expression of REV-ERBα and represses inflammatory factors in THP-1 cells. (F) hLnc-UC overexpression increases REV-ERBα protein and reduces NLRP3, phosphorylated p65 (p-p65), and pro–IL-1β in THP-1 cells. (G) hLnc-UC reduces IL-1β and IL-18 in BMDM incubation medium. (H) Rhythmic expression of hLnc-UC in serum-shocked MDMs. (I) hLnc-UC overexpression induces mRNA expression of REV-ERBα and represses inflammatory factors in MDMs. Data are mean ± SD (n = 5). In (C), (E, left) and (I), *P < 0.05 as determined by Student’s t test; in (E, right) and (G), *P < 0.05 as determined by one-way ANOVA followed by Bonferroni post hoc test.