FIG 6.

Ure2-GFP foci coalesce in cells propagating mutant [URE3] prions. Cells propagating prions composed of wild-type or mutant prions indicated on the left were shifted from medium selecting for prion maintenance to nonselective medium (+ adenine) and monitored at the times indicated at the top. Wild-type and isogenic btn2Δ cur1Δ hsp42Δ cells were monitored similarly. The rightmost column shows fluorescence of [ure-o] cells. The very large bright globules in some T249A wild-type cells at 6 h and K104I triple mutant cells at 0 h are autofluorescing vacuoles, not GFP.