TABLE 1.
Relative anti-[URE3] effects of Ure2p mutations
| Ure2 mutant | [ure-o] frequency (%) for: |
||
|---|---|---|---|
| Dominant inhibitiona | Adenine additionb at: |
||
| t = 0 | 1 day | ||
| None (wild type) | 0 | 0 | 0 |
| N73D | 70 | 4 | 57 |
| K104I | 63 | 5 | 24 |
| S158F | 83 | 1 | 18 |
| R164G | 13 | 1 | 12 |
| H187Q | 29 | 2 | 22 |
| T249A | 7 | 1 | 19 |
| None (empty vector) | 0 | 0 | 0 |
a
Wild-type [URE3] cells were transformed by plasmids encoding the indicated Ure2 mutants. Entirely red colonies were scored as a loss event. The frequency of [URE3] loss reflects the relative “strength” of the inhibitory effect on [URE3]. Values were from roughly 500 transformants.
b
Cells from liquid cultures lacking adenine were tested for the [URE3] phenotype immediately after adding adenine (t = 0) and one day later.