TABLE 2.

Prion seed numbers per cell^a

Strain	Ure2	Genotypeb	Avg no. (SD)	Mutant/WTc	bΔcΔ42Δ/WTd
1976	WT	WT	117 (24)
1977	WT	bΔcΔ42Δ	571 (444)		5
1979	K104I	WT	13 (4)	0.1
1980	K104I	bΔcΔ42Δ	26 (14)		2
1982	S158F	WT	13 (6)	0.1
1983	S158F	bΔcΔ42Δ	107 (42)		9
1985	H187Q	WT	403 (144)	4
1986	H187Q	bΔcΔ42Δ	441 (194)		1
1988	T249A	WT	12 (6)	0.1
1989	T249A	bΔcΔ42Δ	698 (492)		60

^aCells grown without adenine were plated on rich medium containing 3 mM guanidine-HCl and grown to colonies of <1 mm diameter. Five to ten entire colonies of each strain were suspended in water and plated on −ade medium. Values are averages of resulting Ade⁺ colonies (±SD). WT, wild type.

^bbΔcΔ42Δ, btn2::TRP1 cur1::KanMX hsp42::KanMX.

^cRatio of seed numbers in Ure2 mutant cells/117 seeds per cell of Ure2 WT cells.

^dFold increase in avg seed numbers of cells lacking Btn2, Cur1, and Hsp42 versus isogenic WT.