TABLE 3.
Yeast strainsa
| Strain | Relevant genotype | Source or reference |
|---|---|---|
| 1075 | MATa kar1-1 SUQ5 PDAL5::ADE2 his3Δ200 leu2Δ1 trp1Δ63 ura3-52 [URE3] | 62 |
| MR149 | ure2::KanMX | This study |
| 1660 | PRNQ1::URE2-GFP::rnq1 | 35 |
| 1519 | URE2K104I | This study |
| 1520 | URE2R164G | This study |
| 1524 | URE2S158F | This study |
| 1521 | URE2H187Q | This study |
| 1525 | URE2T249A | This study |
| 1975 | btn2::TRP1 cur1::KanMX | This study |
| 1735 | btn2::TRP1 cur1::KanMX URE2K104 | This study |
| 1743 | btn2::TRP1 cur1::KanMX URE2S158F | This study |
| 1739 | btn2::TRP1 cur1::KanMX URE2H187Q | This study |
| 1747 | btn2::TRP1 cur1::KanMX URE2T249A | This study |
| 1976 | PRNQ1::URE2-GFP::rnq1 (1660 backcross) | This study |
| 1979 | 1976 URE2K104I | This study |
| 1982 | 1976 URE2S158 | This study |
| 1985 | 1976 URE2H187Q | This study |
| 1988 | 1976 URE2T249A | This study |
| 1977 | 1976 btn2::TRP1 cur1::KanMX hsp42::KanMX | This study |
| 1980 | 1977 URE2K104I | This study |
| 1983 | 1977 URE2S158F | This study |
| 1986 | 1977 URE2H187Q | This study |
| 1989 | 1977 URE2T249A | This study |
a
All are identical to 1075 except at the indicated loci.