TABLE 4.
Plasmids
| Plasmid | Relevant content | Source or reference |
|---|---|---|
| pRS316 | CEN (single copy), URA3 empty vector | 63 |
| pRS315 | CEN (single copy), LEU2 empty vector | 63 |
| p415 | CEN (single copy), LEU2 empty vector | 64 |
| pKT41 | Bacterial expression vector encoding His6-Ure2 | 6 |
| pROB155 | pRS315 + URE2 (–600 to +500; AatII-XmaI) | This study |
| pEP200 | pRS315 + URE2 (–500 to +300; SacI-SalI) | This study |
| pEP202 | pRS315 + URE2K104I (pEP200 site-directed mutant) | This study |
| pEP211 | pRS315 + URE2S158F (pEP200 site-directed mutant) | This study |
| pEP208 | pRS315 + URE2H187Q (pEP200 site-directed mutant) | This study |
| pEP217 | pRS315 + URE2T249A (pEP200 site-directed mutant) | This study |
| pEAD31 | p415 + PGAL1::URE2 (SpeI-SalI PCR from pEP200) | This study |
| pEAD32 | p415 + PGAL1::URE2K104I (pEAD31site directed mutant) | This study |
| pEAD33 | p415 + PGAL1::URE2S158 (pEAD31site directed mutant) | This study |
| pEAd34 | p415 + PGAL1::URE2H187Q (pEAD31site directed mutant) | This study |
| pEAD35 | p415 + PGAL1::URE2T249A (pEAD31site directed mutant) | This study |
| pEP300 | pKT41 with URE2 (E. coli expression vector) | This study |
| pEP302 | pKT41 with URE2K104I (pEP300 site-directed mutant) | This study |
| pEP311 | pKT41 with URE2S158F (pEP300 site-directed mutant) | This study |
| pEP308 | pKT41 with URE2H187Q (pEP300 site-directed mutant) | This study |
| pEP317 | pKT41 with URE2T249A (pEP300 site-directed mutant) | This study |